File:3D-animation of the diatom Corethron sp.ogv
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Summary[edit]
| Summary | |
|---|---|
| Description | 3D-animation of a Diatom (Corethron sp.) which illustrates how the e-HCFM method supports investigation about microbial interactions
The specimen (surface water, Tara Ocean station 137) was imaged manually from regular e-HCFM sample preparation with a Leica SP8 confocal laser scanning microscope (40X NA1.1 water). Four fluorescent channels are recorded: (i) Green: cellular membranes (DiOC6(3)) indicate the core cell bodies; (ii) Blue: DNA (Hoechst) is used to identify the nucleus; (iii) Red: chlorophyll (autofluorescence); (iv) Grey: PLL-Alexa546 is a useful counterstain for visualizing the specimen surface. 3D reconstruction, surface rendering and animation were conducted with the software Imaris (Bitplane). For further information see: Colin, S., Coelho, L.P., Sunagawa, S., Bowler, C., Karsenti, E., Bork, P., Pepperkok, R. and De Vargas, C. (2017) "Quantitative 3D-imaging for cell biology and ecology of environmental microbial eukaryotes". eLife, 6: e26066. doi:10.7554/eLife.26066.002. |
| Source | Wikimedia Commons file page |
| Author | Sebastien Colin, Luis Pedro Coelho, Shinichi Sunagawa, Chris Bowler, Eric Karsenti, Peer Bork, Rainer Pepperkok, Colomban de Vargas |
| Permission | See original Commons license details. |
Licensing[edit]
Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)
This file is licensed under the Creative Commons Attribution-ShareAlike 4.0 International license.
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Official license: CC BY-SA 4.0
License page: CC BY-SA 4.0
Original attribution and file history: Wikimedia Commons
Summary
| Description |
English: 3D-animation of a Diatom (Corethron sp.) which illustrates how the e-HCFM method supports investigation about microbial interactions The specimen (surface water, Tara Ocean station 137) was imaged manually from regular e-HCFM sample preparation with a Leica SP8 confocal laser scanning microscope (40X NA1.1 water). Four fluorescent channels are recorded: (i) Green: cellular membranes (DiOC6(3)) indicate the core cell bodies; (ii) Blue: DNA (Hoechst) is used to identify the nucleus; (iii) Red: chlorophyll (autofluorescence); (iv) Grey: PLL-Alexa546 is a useful counterstain for visualizing the specimen surface. 3D reconstruction, surface rendering and animation were conducted with the software Imaris (Bitplane).
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| Date | |
| Source | doi:10.7554/eLife.26066.018 |
| Author | Sebastien Colin, Luis Pedro Coelho, Shinichi Sunagawa, Chris Bowler, Eric Karsenti, Peer Bork, Rainer Pepperkok, Colomban de Vargas |
Licensing
- You are free:
- to share – to copy, distribute and transmit the work
- to remix – to adapt the work
- Under the following conditions:
- attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.
File history
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 12:59, 5 October 2020 |  | (11.7 MB) | wikimediacommons>Epipelagic | Uploaded a work by Sebastien Colin, Luis Pedro Coelho, Shinichi Sunagawa, Chris Bowler, Eric Karsenti, Peer Bork, Rainer Pepperkok, Colomban de Vargas from [https://elifesciences.org/articles/26066#fig2video1] {{doi|10.7554/eLife.26066.018}} with UploadWizard |
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