RNA sequencing

RNA sequencing (commonly abbreviated to RNA-seq) is a technology-based methodology that uses next-generation sequencing (NGS) to reveal the presence and quantity of RNA in a biological sample at a given moment. RNA-seq is used to analyze the continuously changing cellular transcriptome.

Overview

RNA-seq is used in transcriptomics, a branch of bioinformatics, and its primary aim is to look at the transcriptome from a high-throughput manner. The transcriptome represents all the RNA molecules, including the mRNA, non-coding RNA, and small RNA, among others, in one cell or a population of cells.

Methodology

The general steps in the RNA-seq process include the initial isolation of RNA, followed by the conversion of isolated RNA to cDNA (complementary DNA). The cDNA is then amplified and sequenced.

Applications

RNA-seq has a wide range of applications, including but not limited to, gene expression profiling, detection of fusion genes, and identification of novel transcripts.

See also

• Transcriptomics technologies
• DNA sequencing
• Next-generation sequencing
• Bioinformatics

References

RNA sequencing Resources
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