Acetone–butanol–ethanol fermentation

Acetone–butanol–ethanol (ABE) fermentation is a process that uses microbial fermentation to produce acetone, butanol, and ethanol from carbohydrates. Initially discovered by Chaim Weizmann in the early 20th century, it was a significant industrial process for the production of solvents during the first half of the 20th century, especially during World War I and World War II. The process utilizes various strains of the bacterium Clostridium acetobutylicum, among others, to ferment sugars into these valuable solvents.

History

ABE fermentation was first developed by Chaim Weizmann, a biochemist who later became the first President of Israel. Weizmann's discovery was pivotal during World War I, as acetone was a critical solvent needed for the production of cordite, a smokeless powder used as a propellant in firearms. The process was industrialized and used extensively in the United Kingdom and other countries to produce acetone for war efforts.

Process

The ABE fermentation process involves two distinct phases: acidogenesis and solventogenesis. During acidogenesis, the microorganisms convert sugars into organic acids, such as acetic acid and butyric acid. As the fermentation progresses and the environment becomes more acidic, the fermentation shifts towards solventogenesis, where the acids are re-assimilated to produce acetone, butanol, and ethanol.

The typical ratio of solvents produced is approximately 3:6:1 (butanol:acetone:ethanol). The process is anaerobic and requires strict control of environmental conditions, including temperature, pH, and nutrient supply, to optimize solvent production and minimize by-product formation.

Microorganisms

The primary microorganisms used in ABE fermentation are from the genus Clostridium, particularly Clostridium acetobutylicum. These bacteria are anaerobic, spore-forming, and capable of fermenting a wide range of carbohydrates, including glucose, fructose, and xylose, among others. Genetic engineering and strain improvement efforts have been directed at enhancing solvent yields, tolerance to high solvent concentrations, and substrate range.

Applications

The solvents produced through ABE fermentation have a wide range of industrial applications. Acetone and butanol are important solvents in the chemical industry, used in the manufacture of plastics, pharmaceuticals, and cosmetics. Ethanol is a widely used solvent and fuel additive. Despite the decline in the ABE fermentation process with the advent of petrochemical methods for solvent production, there has been renewed interest in this biological process due to the increasing demand for sustainable and renewable chemical production methods.

Challenges and Future Directions

The economic viability of ABE fermentation has been challenged by the high cost of substrate, low solvent yields, and product inhibition. Research is ongoing to overcome these challenges through the development of more robust microbial strains, utilization of cheaper substrates such as lignocellulosic biomass, and improvements in fermentation technology. Advances in biotechnology and genetic engineering hold promise for the revitalization of this once-dominant industrial process, contributing to a more sustainable and environmentally friendly chemical industry.

Acetone–butanol–ethanol fermentation Resources
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