Aniline blue

Aniline Blue is a synthetic dye derived from aniline, a type of organic compound. It is primarily used in the staining of biological samples for microscopic examination, particularly in the field of histology.

History

Aniline Blue was first synthesized in the mid-19th century, during a period of rapid development in the field of synthetic dyes. The discovery of aniline and its derivatives marked a significant advancement in the dye industry, leading to the production of a wide range of colors previously unattainable with natural dyes.

Chemical Properties

Aniline Blue is a member of the triphenylmethane family of dyes. It is a complex organic molecule, with a structure that includes three phenyl rings attached to a central carbon atom. The dye is soluble in water and alcohol, and its color varies from blue to green depending on the pH of the solution.

Uses

In histology, Aniline Blue is used to stain collagen and other connective tissues. It is a component of several staining protocols, including the Masson's trichrome and the Mallory's trichrome stains. In these protocols, Aniline Blue is used in combination with other dyes to provide a contrast between different tissue types.

In addition to its use in histology, Aniline Blue is also used in the textile industry as a dye for fabrics. However, its use in this context has declined in recent years due to concerns about the environmental impact of synthetic dyes.

Safety and Environmental Concerns

Like many synthetic dyes, Aniline Blue is associated with a number of safety and environmental concerns. It is toxic if ingested or inhaled, and it can cause skin and eye irritation. In the environment, Aniline Blue can be harmful to aquatic life.

See Also

• Aniline
• Histology
• Triphenylmethane dyes