CCRF-CEM

Template:Infobox cell line CCRF-CEM is a human T lymphoblast cell line derived from the peripheral blood of a child with acute lymphoblastic leukemia (ALL). This cell line is widely used in cancer research and drug development due to its well-characterized genetic and phenotypic properties.

History

The CCRF-CEM cell line was established in 1964 by researchers at the Children's Cancer Research Foundation (CCRF). The cell line was derived from the peripheral blood of a female child diagnosed with acute lymphoblastic leukemia. Since its establishment, CCRF-CEM has been extensively used in various scientific studies to understand the biology of leukemia and to develop new therapeutic strategies.

Characteristics

CCRF-CEM cells exhibit the typical characteristics of T lymphoblasts, including a high nucleus-to-cytoplasm ratio and the presence of surface markers such as CD3, CD4, and CD7. These cells are known for their rapid proliferation and ability to grow in suspension culture. The karyotype of CCRF-CEM cells is complex, with multiple chromosomal abnormalities commonly associated with leukemia.

Applications

CCRF-CEM cells are utilized in a wide range of research applications, including:

• Cancer research: Studying the molecular mechanisms underlying leukemia and other cancers.
• Drug development: Screening and evaluating the efficacy of new anti-cancer drugs.
• Genetic studies: Investigating the genetic alterations and pathways involved in leukemia.

Related Cell Lines

Several sublines and related cell lines have been derived from CCRF-CEM, including:

• CEM-C1
• CEM-C7
• CEM-CCRF

See Also

• Acute lymphoblastic leukemia
• Cancer cell lines
• Drug development
• T lymphoblast