Cytochalasin B

Cytochalasin B

Chemical structure of Cytochalasin B

Cytochalasin B is a natural product that belongs to the class of fungal metabolites known as cytochalasins. It was first isolated from the fungus Helminthosporium dematioideum in 1967. Cytochalasin B is widely used in biological research as a tool to study actin dynamics and cell motility.

Chemical Structure

Cytochalasin B has a complex chemical structure consisting of a macrocyclic lactone ring fused to a dihydroisocoumarin moiety. It is characterized by a 13-membered ring containing a tetrahydrofuran and a tetrahydropyran ring. The lactone ring is responsible for the biological activity of Cytochalasin B.

Biological Activity

Cytochalasin B is a potent inhibitor of actin polymerization. It binds to the barbed end of actin filaments, preventing the addition of monomers and thus inhibiting filament elongation. This disruption of actin dynamics leads to the inhibition of various cellular processes that depend on actin, such as cell migration, cytokinesis, and endocytosis.

Applications

Cytochalasin B has been widely used in cell biology research to investigate the role of actin in various cellular processes. It has been used to study cell motility, cell division, and intracellular trafficking. Additionally, Cytochalasin B has been used as a tool to investigate the mechanism of action of other actin-binding proteins and drugs.

See Also

• Actin
• Cell Motility
• Cytokinesis
• Endocytosis