DPX

DPX

DPX is a term that can refer to several different concepts in the medical and scientific fields. This article will focus on DPX as it relates to histology and microscopy, specifically as a mounting medium used in the preparation of microscope slides.

Overview

DPX is a synthetic resin-based mounting medium commonly used in histology and microscopy for the preparation of permanent slides. It is known for its ability to preserve specimens and provide a clear, durable finish that enhances the visibility of the sample under a microscope.

Composition

DPX is composed of a mixture of distyrene, a plasticizer, and xylene. The combination of these components results in a medium that is both adhesive and optically clear, making it ideal for mounting biological specimens.

Distyrene

Distyrene is a synthetic polymer that provides the structural backbone of the DPX medium. It contributes to the rigidity and stability of the mounted specimen.

Plasticizer

The plasticizer in DPX helps to maintain the flexibility and workability of the medium, preventing it from becoming too brittle over time.

Xylene

Xylene is an organic solvent that acts as a carrier for the distyrene and plasticizer. It evaporates as the DPX dries, leaving behind a clear, solid film.

Applications

DPX is primarily used in the field of histology for mounting tissue sections that have been stained and are ready for microscopic examination. It is also used in other areas of microscopy where permanent slide preparation is required.

Advantages

- Clarity: DPX provides excellent optical clarity, which is essential for detailed microscopic analysis. - Durability: Once dried, DPX forms a hard, protective layer that preserves the specimen for long-term storage and repeated viewing. - Compatibility: It is compatible with a wide range of stains and biological materials.

Disadvantages

- Toxicity: Xylene, a component of DPX, is toxic and requires careful handling and adequate ventilation during use. - Drying Time: DPX can take several hours to fully dry and harden, which may be a limitation in time-sensitive applications.

Preparation and Usage

To use DPX as a mounting medium, a small amount is applied to the specimen on the slide, and a coverslip is carefully placed on top. The slide is then allowed to dry, during which the xylene evaporates, leaving a clear, solidified layer.

Safety Considerations

Due to the presence of xylene, it is important to use DPX in a well-ventilated area and to wear appropriate personal protective equipment, such as gloves and goggles, to minimize exposure.

Also see

- Histology - Microscopy - Mounting medium - Xylene