DTNB
DTNB or 5,5'-Dithiobis(2-nitrobenzoic acid) is a chemical reagent often used in scientific research. It is also known by the name Ellman's reagent. DTNB is a compound that measures the concentration of thiol groups in a solution.
Chemical Properties[edit | edit source]
DTNB is a yellow crystalline powder that is soluble in water and alcohol. It has a molecular weight of 396.35 g/mol and a melting point of 198-200°C. The chemical formula for DTNB is C14H8N2O8S2.
Uses[edit | edit source]
DTNB is primarily used in biochemistry for the quantification of protein thiols. It reacts with thiols to form a mixed disulfide and 2-nitro-5-thiobenzoate (TNB), which is a yellow compound that can be detected spectrophotometrically at 412 nm. This reaction is the basis for the Ellman's test, a common method for quantifying free thiols in solution.
Safety[edit | edit source]
DTNB is considered a low hazard compound. However, it can cause skin and eye irritation, and should be handled with care. It should be stored in a cool, dry place, away from light.
See Also[edit | edit source]
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Contributors: Prab R. Tumpati, MD
