Eluate

Eluate is a solution obtained from the process of elution. Elution is the process of extracting one material from another by washing with a solvent to remove adsorbed material from an adsorbent (as in washing of loaded ion-exchange resins to remove captured ions).

Process

In a typical chromatography process, the stationary phase is packed in a column, and the mobile phase is forced through the column. The individual components of the mixture are flushed through the column at different rates, depending on their affinity to the stationary phase. The time at which a specific analyte elutes (emerges from the column) is called its retention time. The retention time measured under particular conditions is considered an identifying characteristic of a given analyte.

Applications

Eluates are commonly used in biochemistry and molecular biology. For example, in immunology, an eluate can be used to extract antibodies from a cell or to separate a mixture of proteins or nucleic acids.

See also

• Chromatography
• Biochemistry
• Molecular biology
• Immunology
• Protein
• Nucleic acid

Eluate Resources
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