Elute
Elute is a term commonly used in chemistry and biochemistry to describe the process of extracting one material from another by washing with a solvent. This process is often used in chromatography to separate different components of a mixture.
Etymology[edit | edit source]
The word "elute" is derived from the Latin word elutus, the past participle of eluere, which means "to wash out."
Applications[edit | edit source]
Elution is a critical step in various scientific techniques, including:
- Chromatography: In liquid chromatography, elution is the process of passing a liquid or gas through a column to separate different substances based on their interactions with the stationary phase.
- Protein purification: Elution is used to separate proteins from a mixture, often using a gradient of increasing ionic strength or pH.
- DNA extraction: Elution is used to purify DNA from a sample, typically involving the use of a buffer to wash the DNA off a solid support.
Types of Elution[edit | edit source]
There are several types of elution techniques, including:
- Isocratic elution: The composition of the solvent remains constant throughout the process.
- Gradient elution: The composition of the solvent changes gradually, often becoming more polar or less polar over time.
Elution in Chromatography[edit | edit source]
In chromatography, elution is a key step that determines the separation of components. The elution process can be influenced by several factors, including:
- The nature of the stationary phase
- The composition of the mobile phase
- The flow rate of the mobile phase
- The temperature of the system
Elution Profile[edit | edit source]
An elution profile is a graphical representation of the elution process, showing the concentration of different components as a function of time or volume of eluent. This profile helps in identifying and quantifying the separated components.
Related Techniques[edit | edit source]
- Adsorption
- Desorption
- Affinity chromatography
- Ion exchange chromatography
- Gel filtration chromatography
See Also[edit | edit source]
References[edit | edit source]
External Links[edit | edit source]
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