Gene library

Gene library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a gene library, the organism's DNA is extracted from cells and then digested into fragments that can be inserted into the vector.

Overview

The term "gene library" can be used in two ways. In the broad sense, a gene library is a collection of DNA fragments that represent the entire genome of an organism. In a more narrow sense, a gene library can refer to a collection of DNA fragments that have been cloned (copied) into a vector so they can be more easily studied.

Construction of a Gene Library

The construction of a gene library involves several steps. First, the DNA is extracted from the organism. This DNA is then cut into fragments using restriction enzymes. These fragments are then inserted into a vector, which is usually a plasmid or a virus. The vector is then introduced into a host organism, such as a bacterium. The host organism replicates the vector, creating many copies of the DNA fragment.

Uses of a Gene Library

Gene libraries are used in many areas of biological research. They can be used to study the function of specific genes, to produce proteins, or to create genetically modified organisms. They can also be used in the field of genomics to sequence genomes and to study the genetic variation in a population.

See Also

• Genomics
• Genetic engineering
• DNA sequencing
• Molecular cloning

References

Gene library Resources
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