HNCOCA experiment

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== HNCOCA Experiment ==

The HNCOCA experiment is a type of NMR spectroscopy experiment commonly used in the field of structural biology. This experiment is particularly useful for studying the backbone amino acid residues in proteins.

Principle

In the HNCOCA experiment, the magnetization transfer pathway involves the nitrogen, carbon, and alpha carbon atoms of the amino acid residues. This experiment allows for the correlation of the nitrogen and alpha carbon chemical shifts, providing valuable information for determining the protein structure.

Procedure

During the HNCOCA experiment, a series of pulse sequences are applied to the protein sample in the NMR spectrometer. These pulse sequences selectively manipulate the magnetization of the nitrogen and carbon nuclei, leading to the observation of cross peaks in the resulting spectrum.

Applications

The HNCOCA experiment is widely used in protein NMR spectroscopy studies to assign the backbone resonances of proteins and to obtain structural information. By analyzing the cross peaks in the spectrum, researchers can determine the connectivity between adjacent amino acid residues in the protein sequence.

See also

• Nuclear magnetic resonance spectroscopy
• Protein structure
• Pulse sequence