LNCaP

LNCaP

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Comparison of cancer cell lines

File:Thalmann figure.png

Thalmann figure

LNCaP (Lymph Node Carcinoma of the Prostate) is a human prostate cancer cell line commonly used in cancer research. It was established from a metastatic lesion of human prostatic adenocarcinoma in the left supraclavicular lymph node of a 50-year-old Caucasian male. LNCaP cells are androgen-sensitive, meaning their growth is stimulated by androgens, which are male hormones such as testosterone.

Characteristics

LNCaP cells exhibit several characteristics that make them valuable for research:

• They express prostate-specific antigen (PSA), a protein produced by both normal and malignant cells of the prostate gland.
• They possess a mutated androgen receptor (AR), which allows them to respond to lower levels of androgens compared to normal prostate cells.
• They are used to study the mechanisms of androgen receptor signaling and the development of androgen-independent prostate cancer.

Research Applications

LNCaP cells are widely used in various areas of prostate cancer research, including:

• Drug discovery: Testing the efficacy of new therapeutic agents.
• Gene expression studies: Understanding the role of specific genes in prostate cancer progression.
• Signal transduction pathways: Investigating how signals are transmitted within cells to promote cancer growth.

Culturing LNCaP Cells

LNCaP cells are typically cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. They are maintained at 37°C in a humidified atmosphere containing 5% carbon dioxide (CO2).

Related Cell Lines

Other prostate cancer cell lines used in research include:

• PC-3
• DU145
• 22Rv1

See Also

• Prostate cancer
• Androgen receptor
• Prostate-specific antigen
• Cancer cell line
• Drug discovery