LNCaP
LNCaP (Lymph Node Carcinoma of the Prostate) is a human prostate cancer cell line commonly used in cancer research. It was established from a metastatic lesion of human prostatic adenocarcinoma in the left supraclavicular lymph node of a 50-year-old Caucasian male. LNCaP cells are androgen-sensitive, meaning their growth is stimulated by androgens, which are male hormones such as testosterone.
Characteristics[edit | edit source]
LNCaP cells exhibit several characteristics that make them valuable for research:
- They express prostate-specific antigen (PSA), a protein produced by both normal and malignant cells of the prostate gland.
- They possess a mutated androgen receptor (AR), which allows them to respond to lower levels of androgens compared to normal prostate cells.
- They are used to study the mechanisms of androgen receptor signaling and the development of androgen-independent prostate cancer.
Research Applications[edit | edit source]
LNCaP cells are widely used in various areas of prostate cancer research, including:
- Drug discovery: Testing the efficacy of new therapeutic agents.
- Gene expression studies: Understanding the role of specific genes in prostate cancer progression.
- Signal transduction pathways: Investigating how signals are transmitted within cells to promote cancer growth.
Culturing LNCaP Cells[edit | edit source]
LNCaP cells are typically cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. They are maintained at 37°C in a humidified atmosphere containing 5% carbon dioxide (CO2).
Related Cell Lines[edit | edit source]
Other prostate cancer cell lines used in research include:
See Also[edit | edit source]
References[edit | edit source]
External Links[edit | edit source]
Navigation: Wellness - Encyclopedia - Health topics - Disease Index - Drugs - World Directory - Gray's Anatomy - Keto diet - Recipes
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD
WikiMD is not a substitute for professional medical advice. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Contributors: Prab R. Tumpati, MD