Methyl green

Methyl Green is a chemical compound used as a stain in histology. It is part of the trihphenylmethane dye family and is used to stain nuclei in tissue samples.

History

Methyl Green was first synthesized in the late 19th century and has been used in histological staining since the early 20th century. It is often used in combination with other stains, such as pyronin, to provide contrast and highlight specific structures within tissue samples.

Chemical Structure

Methyl Green is a triphenylmethane dye, meaning it has three phenyl groups attached to a central carbon atom. The molecule also contains a nitrogen atom, which is responsible for its ability to bind to DNA and stain nuclei.

Use in Histology

In histology, Methyl Green is used to stain nuclei in tissue samples. It binds to DNA, causing the nuclei to appear green under a microscope. This allows researchers to easily identify and study the nuclei within cells. Methyl Green is often used in combination with other stains, such as pyronin, which stains RNA red, to provide contrast and highlight specific structures within tissue samples.

Safety and Handling

Like many chemical compounds, Methyl Green should be handled with care. It can be harmful if swallowed or inhaled, and it can cause skin and eye irritation. Proper safety equipment, including gloves and eye protection, should be used when handling Methyl Green.

See Also

• Histology
• Stain
• Triphenylmethane dye
• DNA
• RNA

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