Methylene green
Methylene Green is a synthetic dye used in various scientific and medical applications. Its chemical structure and properties make it useful for staining cells and tissues, facilitating the visualization of cellular components under a microscope. Methylene Green is closely related to other thiazine dyes, sharing similarities with Methylene Blue, but it has unique applications in the field of histology and cytology.
Chemical Properties[edit | edit source]
Methylene Green, chemically known as tetramethylthionine chloride, is a thiazine dye. It has a distinctive green color and is soluble in water and alcohol. The dye binds to acidic components of cells, making it an ideal stain for highlighting nucleic acids and other acidic cellular components.
Applications[edit | edit source]
Histology[edit | edit source]
In histology, Methylene Green is used to stain the nuclei of cells, making it easier to distinguish them from the surrounding cytoplasm. This staining technique is particularly useful in the study of cell structure and the identification of abnormal or cancerous cells.
Cytology[edit | edit source]
In cytology, Methylene Green is applied to samples to visualize cells more clearly under a microscope. It is often used in combination with other dyes, such as Eosin, to provide contrast and enhance the visualization of cellular details.
Molecular Biology[edit | edit source]
Methylene Green has applications in molecular biology as well. It can be used as a non-radioactive marker for tracking the migration of nucleic acids in gel electrophoresis, aiding in the study of genetic materials.
Safety and Handling[edit | edit source]
While Methylene Green is a valuable tool in scientific research, it must be handled with care. It is important to wear appropriate protective equipment and follow safety guidelines to avoid exposure and potential health risks.
See Also[edit | edit source]
References[edit | edit source]
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD
|
WikiMD's Wellness Encyclopedia |
| Let Food Be Thy Medicine Medicine Thy Food - Hippocrates |
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Medical Disclaimer: WikiMD is not a substitute for professional medical advice. The information on WikiMD is provided as an information resource only, may be incorrect, outdated or misleading, and is not to be used or relied on for any diagnostic or treatment purposes. Please consult your health care provider before making any healthcare decisions or for guidance about a specific medical condition. WikiMD expressly disclaims responsibility, and shall have no liability, for any damages, loss, injury, or liability whatsoever suffered as a result of your reliance on the information contained in this site. By visiting this site you agree to the foregoing terms and conditions, which may from time to time be changed or supplemented by WikiMD. If you do not agree to the foregoing terms and conditions, you should not enter or use this site. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates, categories Wikipedia, licensed under CC BY SA or similar.
Contributors: Prab R. Tumpati, MD
