N-ethylmaleimide sensitive fusion protein

Protein NSF PDB 1d2n

N-ethylmaleimide sensitive fusion protein

The N-ethylmaleimide sensitive fusion protein (NSF) is a key component in the process of membrane fusion within cells. It is a type of ATPase enzyme that plays a crucial role in vesicle trafficking and fusion events in eukaryotic cells.

Structure

NSF is a hexameric protein complex composed of subunits that contain both ATPase and SNARE-binding domains. The ATPase activity of NSF provides the energy required for the disassembly of SNARE complexes during membrane fusion.

Function

NSF functions by binding to soluble NSF attachment proteins (SNAPs) and SNARE proteins on opposing membranes. This interaction facilitates the disassembly of SNARE complexes, allowing membranes to fuse and cargo to be delivered to their target destinations within the cell.

Regulation

The activity of NSF is tightly regulated by various factors, including the availability of ATP and the presence of regulatory proteins. Dysregulation of NSF activity can lead to defects in membrane trafficking and cellular function.

Role in Cellular Processes

NSF is involved in a wide range of cellular processes, including neurotransmitter release, hormone secretion, and intracellular membrane trafficking. It is essential for maintaining the integrity and functionality of cellular compartments.

Clinical Significance

Mutations in the gene encoding NSF have been associated with certain neurological disorders and neurodegenerative diseases. Understanding the role of NSF in cellular processes is important for developing potential therapeutic interventions for these conditions.

See Also

• ATPase
• Membrane Fusion
• Vesicle Trafficking