Ouchterlony
Ouchterlony double immunodiffusion (also known as Ouchterlony double diffusion) is a method of immunodiffusion in agar gel that was introduced by the Swedish physician Örjan Ouchterlony in 1948. This method is used to test the antigen-antibody reactions.
Principle[edit | edit source]
The principle of Ouchterlony double immunodiffusion involves the diffusion of antigens and antibodies through a semi-solid medium, which is usually agar or agarose gel. The antigens and antibodies diffuse towards each other and when they meet, they form a line of precipitation, which is visible to the naked eye.
Procedure[edit | edit source]
The procedure of Ouchterlony double immunodiffusion involves the following steps:
- A layer of agar gel is poured onto a glass slide or petri dish.
- Wells are cut into the agar gel using a template.
- The antigen is placed in the central well and the antibodies are placed in the surrounding wells.
- The slide or dish is incubated at room temperature for 24-48 hours.
- The antigen and antibodies diffuse out of the wells and into the agar gel.
- When the antigen and antibodies meet, they form a line of precipitation.
Applications[edit | edit source]
Ouchterlony double immunodiffusion is used in various fields of medical and biological research. Some of the applications of this method include:
- Detection and identification of antigens or antibodies.
- Determination of the relative concentration of antigens or antibodies.
- Determination of the specificity of antigens or antibodies.
- Determination of the purity of antigens or antibodies.
See also[edit | edit source]
References[edit | edit source]
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