Recombinant DNA technology

Recombinant DNA technology (rDNA) is a series of procedures that are used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed from segments of two or more different DNA molecules. Under certain conditions, a recombinant DNA molecule can enter a cell and replicate there, either autonomously or after it has been integrated into a cellular chromosome.

History

Recombinant DNA technology was first proposed by Peter Lobban, a graduate student, and A. Dale Kaiser, a professor, both at Stanford University, in a July 1973 paper in the Journal of Molecular Biology.

Process

The process of creating recombinant DNA involves several steps: isolation of the DNA to be recombined, cutting of the DNA into smaller fragments, joining of these fragments with another molecule of DNA, and finally, transformation of this new DNA into an organism.

Applications

Recombinant DNA technology has been used to create different types of medicines and vaccines, to diagnose diseases, to produce industrial goods, and to genetically modify crops.

See also

• Genetic engineering
• Molecular cloning
• Polymerase chain reaction

External links

• Original paper proposing the technique

Recombinant DNA technology Resources
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