8-Oxo-2'-deoxyguanosine

8-Oxo-2'-deoxyguanosine (also known as 8-oxo-dG, 8-oxo-2'-dG or 8-hydroxy-2'-deoxyguanosine) is a nucleoside that is a derivative of deoxyguanosine. It is one of the major products of DNA oxidation, and is often used as a marker for DNA damage and oxidative stress.

Structure and Properties

8-Oxo-2'-deoxyguanosine consists of a guanine base attached to a deoxyribose sugar. The guanine base is modified by the addition of an oxygen atom at the 8th position, resulting in the formation of 8-oxo-guanine. This modification significantly alters the properties of the nucleoside, including its ability to form hydrogen bonds and its base pairing behavior.

Biological Significance

8-Oxo-2'-deoxyguanosine is a major product of DNA oxidation, and its presence in DNA can lead to mutations and genetic instability. This is because 8-oxo-guanine can mispair with adenine during DNA replication, leading to G to T transversion mutations. These mutations can contribute to the development of various diseases, including cancer and neurodegenerative diseases.

The levels of 8-oxo-2'-deoxyguanosine in DNA can be increased by various factors, including oxidative stress, inflammation, and exposure to ionizing radiation or certain chemicals. Therefore, the measurement of 8-oxo-2'-deoxyguanosine levels is often used as a marker for DNA damage and oxidative stress.

Detection and Measurement

8-Oxo-2'-deoxyguanosine can be detected and measured using various techniques, including high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA), and mass spectrometry. These techniques allow for the quantification of 8-oxo-2'-deoxyguanosine in DNA, and can be used to assess the extent of DNA damage and oxidative stress in cells and tissues.

See Also

• 8-Oxoguanine
• Oxidative stress
• DNA damage (naturally occurring)
• DNA repair

References

Y-STR Name

Haplotype

‎

---

Translate: - East Asian 中文, 日本, 한국어, South Asian हिन्दी, தமிழ், తెలుగు, Urdu, ಕನ್ನಡ, Southeast Asian Indonesian, Vietnamese, Thai, မြန်မာဘာသာ, বাংলা
European español, Deutsch, français, Greek, português do Brasil, polski, română, русский, Nederlands, norsk, svenska, suomi, Italian
Middle Eastern & African عربى, Turkish, Persian, Hebrew, Afrikaans, isiZulu, Kiswahili,
Other Bulgarian, Hungarian, Czech, Swedish, മലയാളം, मराठी, ਪੰਜਾਬੀ, ગુજરાતી, Portuguese, Ukrainian