DEPT (medicine)

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DEPT (Distortionless Enhancement by Polarization Transfer) is a nuclear magnetic resonance (NMR) technique commonly used in medicine and biochemistry to enhance signal intensity. It is particularly useful in the study of organic compounds and proteins.

Overview[edit | edit source]

DEPT is a polarization transfer technique that selectively excites certain types of hydrogen atoms in a sample, enhancing their signals in the resulting NMR spectrum. This allows for more detailed analysis of the molecular structure of the sample.

The technique involves the use of a series of radiofrequency pulses and magnetic field gradients to selectively excite and then transfer polarization from carbon-13 nuclei to attached protons. The resulting signal enhancement allows for more detailed analysis of the molecular structure of the sample.

Applications[edit | edit source]

In medicine, DEPT is often used in magnetic resonance imaging (MRI) to enhance the contrast between different tissues, making it easier to identify abnormalities. It is also used in biochemical research to study the structure of proteins and other complex molecules.

In addition to its use in medicine and biochemistry, DEPT is also used in chemistry to study the structure of organic compounds. By selectively exciting certain types of hydrogen atoms, it allows for more detailed analysis of the molecular structure of the compound.

See also[edit | edit source]


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Contributors: Prab R. Tumpati, MD