Bromphenol blue
Bromphenol Blue is a chemical compound used as a pH indicator in molecular biology laboratories. It is a member of the class of compounds known as triphenylmethane dyes, which are used in a variety of scientific and industrial applications.
Chemical Properties[edit | edit source]
Bromphenol blue is a dark purple crystalline powder that is soluble in water and alcohol. It has a molecular formula of C19H10Br4O5S and a molecular weight of 669.96 g/mol. The compound has a pKa value of 4.0, which means it can act as an acid in solution.
Uses[edit | edit source]
Bromphenol blue is primarily used as a tracking dye for DNA/RNA in agarose gel electrophoresis. It is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis. In addition, bromphenol blue is used as a pH indicator, changing from yellow at pH 3.0 to purple at pH 4.6.
Safety[edit | edit source]
Bromphenol blue is considered a low hazard compound. However, it can cause irritation to the eyes, skin, and respiratory tract. It is recommended to handle the compound with appropriate protective clothing and in a well-ventilated area.
See Also[edit | edit source]
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD
|
WikiMD's Wellness Encyclopedia |
| Let Food Be Thy Medicine Medicine Thy Food - Hippocrates |
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Medical Disclaimer: WikiMD is not a substitute for professional medical advice. The information on WikiMD is provided as an information resource only, may be incorrect, outdated or misleading, and is not to be used or relied on for any diagnostic or treatment purposes. Please consult your health care provider before making any healthcare decisions or for guidance about a specific medical condition. WikiMD expressly disclaims responsibility, and shall have no liability, for any damages, loss, injury, or liability whatsoever suffered as a result of your reliance on the information contained in this site. By visiting this site you agree to the foregoing terms and conditions, which may from time to time be changed or supplemented by WikiMD. If you do not agree to the foregoing terms and conditions, you should not enter or use this site. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.
Contributors: Prab R. Tumpati, MD
