Cibacron blue

{{Infobox chemical | name = Cibacron Blue | image = Cibacron_Blue_Structure.png | image_size = 250px | image_caption = Chemical structure of Cibacron Blue | IUPAC_name = 2-Anthracenesulfonic acid, 1-amino-9,10-dihydro-9,10-dioxo-4-[[3-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]amino]- | other_names = Reactive Blue 2 | CAS_number = 2650-18-2 | molecular_formula = C29H20N2O11S3 | molar_mass = 678.66 g/mol }}

Cibacron Blue, also known as Reactive Blue 2, is a synthetic dye commonly used in biochemistry and molecular biology for its ability to bind to proteins, particularly albumin and enzymes such as dehydrogenases. It is a member of the anthraquinone family of dyes and is characterized by its vivid blue color.

Chemical Properties

Cibacron Blue is an anthraquinone dye with a complex structure that includes sulfonic acid groups, which confer water solubility, and reactive groups that allow it to form covalent bonds with proteins. Its molecular formula is C29H20N2O11S3, and it has a molar mass of 678.66 g/mol.

Applications in Biochemistry

Cibacron Blue is widely used in affinity chromatography due to its ability to mimic the structure of nucleotides, allowing it to bind to nucleotide-binding sites on proteins. This property makes it particularly useful for purifying kinases, dehydrogenases, and other nucleotide-dependent enzymes.

Affinity Chromatography

In affinity chromatography, Cibacron Blue is immobilized on a solid support, such as agarose beads, to create a column matrix. Proteins with affinity for the dye are retained on the column, while other proteins are washed away. The bound proteins can then be eluted using a high concentration of a competing ligand, such as ATP or NADH.

Protein Purification

Cibacron Blue is used to purify serum albumin and other proteins from complex mixtures. Its ability to bind to a wide range of proteins makes it a versatile tool in protein purification protocols.

Safety and Handling

As with many synthetic dyes, Cibacron Blue should be handled with care. It is important to use appropriate personal protective equipment, such as gloves and goggles, when working with this chemical. It should be stored in a cool, dry place, away from incompatible substances.

Also see

• Affinity chromatography
• Protein purification
• Serum albumin
• Nucleotide
• Enzyme

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This article is a stub related to biochemistry. You can help WikiMD by expanding it!