Cresolphthalein
Cresolphthalein is a chemical compound used as a pH indicator in biological and medical laboratories. It is a triphenylmethane dye, which changes color from colorless to purple as the pH of a solution increases.
Chemical Properties[edit | edit source]
Cresolphthalein is a white to pale-yellow crystalline powder. It is soluble in alcohol and slightly soluble in water. The chemical formula of cresolphthalein is C22H18O4. It has a molecular weight of 354.37 g/mol.
Uses[edit | edit source]
Cresolphthalein is primarily used as a pH indicator in various laboratory tests. It is also used in the medical field for the determination of calcium in serum. The cresolphthalein complexone method is a popular method for serum calcium determination.
Cresolphthalein Complexone Method[edit | edit source]
The cresolphthalein complexone method is a colorimetric method used for the determination of calcium in serum. In this method, cresolphthalein forms a purple complex with calcium in alkaline solution. The intensity of the purple color is directly proportional to the calcium concentration in the solution.
Safety[edit | edit source]
Cresolphthalein is not classified as a hazardous substance. However, it may cause irritation to the eyes, skin, and respiratory tract. It is recommended to handle cresolphthalein with appropriate protective equipment.
See Also[edit | edit source]
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Contributors: Prab R. Tumpati, MD
