Dialysis (biochemistry)

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Dialysis (biochemistry)

Dialysis (biochemistry) is a process used in biochemistry to separate molecules based on their size and shape. This process is often used to remove unwanted small molecules from a solution of larger molecules.

Process[edit | edit source]

Dialysis works by diffusion, a process where molecules move from an area of high concentration to an area of low concentration. In dialysis, a semi-permeable membrane is used to separate the small molecules from the larger ones. The small molecules pass through the membrane, while the larger molecules are retained.

File:Dialysis (biochemistry).jpg
A diagram showing the process of dialysis in biochemistry.

Uses[edit | edit source]

Dialysis is used in a variety of applications in biochemistry. It is often used to remove salts or other small molecules from a solution of larger molecules, such as proteins. This can be important in the preparation of samples for further analysis or use.

Types of Dialysis[edit | edit source]

There are several types of dialysis used in biochemistry, including:

  • Continuous dialysis: This is a type of dialysis where the dialysis solution is continuously replaced. This can be useful for removing a large amount of small molecules from a solution.
  • Equilibrium dialysis: This is a type of dialysis where the solution is allowed to reach equilibrium, meaning that the concentration of small molecules is the same on both sides of the membrane.
  • Microdialysis: This is a type of dialysis that uses a small dialysis membrane. This can be useful for analyzing small samples.

See Also[edit | edit source]


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Contributors: Prab R. Tumpati, MD