Dichlorophenolindophenol

Dichlorophenolindophenol (DCPIP) is a chemical compound commonly used as a redox dye. When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless.

Chemical Properties

DCPIP can serve as a redox indicator that is blue when oxidized and colorless when reduced. This property makes it useful in experiments that involve redox reactions, particularly in biochemistry. For example, it can be used to measure the rate of reaction in the electron transport chain.

Uses

One of the main uses of DCPIP is as a redox indicator in biochemical experiments, particularly those involving the electron transport chain. It is also used in the vitamin C assay, where it changes from blue to colorless upon reduction by ascorbic acid.

Health Effects

Exposure to DCPIP can cause irritation to the skin, eyes, and respiratory tract. It may also cause allergic skin reactions. Ingestion can lead to gastrointestinal irritation, nausea, vomiting, and diarrhea.

Safety

When handling DCPIP, it is important to wear appropriate protective clothing and eye/face protection. In case of inadequate ventilation, wear respiratory protection. If swallowed, seek medical advice immediately and show the container or label.

See Also

• Redox indicator
• Electron transport chain
• Vitamin C