Genomic library

Genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested into fragments that can be inserted into the vector.

Overview

The genomic library contains all the sequences in the entire genome of an organism. It is different from a cDNA library in that it includes exons, introns, and noncoding sequences. The genomic library is a valuable tool in molecular biology research, as it allows for the study of gene structure, function, and evolution.

Construction

The construction of a genomic library involves several steps. First, the genomic DNA is extracted from the organism. This DNA is then fragmented, either by using restriction enzymes or by physical shearing. The resulting fragments are then inserted into a suitable vector. The vector is then introduced into a host organism, such as a bacterium, where it can replicate to produce many copies of the inserted DNA.

Applications

Genomic libraries are used in a variety of research applications. They can be used to map the genome of an organism, to identify and study specific genes, and to investigate the function of unknown genes. They are also used in comparative genomics, to compare the genomes of different species.

See also

• Molecular cloning
• Genome project
• Genetic engineering
• DNA sequencing

References

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  Genomic Library Construction
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  Blot Hybridization
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  BAC Vector
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  Whole Genome Shotgun Sequencing versus Hierarchical Shotgun Sequencing