Hanes–Woolf plot

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Hanes–Woolf plot - brief summary


In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of against , is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration to the reaction velocity is plotted against. It is based on the rearrangement of the Michaelis–Menten equation shown below: where is the Michaelis constant and is the limiting rate. Starting from the Michaelis–Menten equation: we can take reciprocals of both sides of the equation to obtain the equation underlying the Lineweaver–Burk plot: · which can be rearranged to express a different straight-line relationship: ·.

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