LacUV5

LacUV5 is a promoter in the lac operon that is responsible for the regulation of gene expression in bacteria. It is a variant of the wild-type lac promoter, and is known for its high level of activity and resistance to catabolite repression.

Overview

The LacUV5 promoter is a mutation of the lac promoter that results in a higher level of transcription than the wild-type promoter. This is due to changes in the -10 and -35 regions of the promoter, which increase the binding affinity of RNA polymerase and lead to increased transcription initiation.

The LacUV5 promoter is also resistant to catabolite repression, a process that normally inhibits the expression of genes involved in the metabolism of alternative sugars when glucose is present. This resistance is due to the fact that the LacUV5 promoter is not recognized by the catabolite activator protein (CAP), a protein that normally enhances the activity of the lac promoter in the presence of cAMP.

Structure and Function

The LacUV5 promoter consists of two main regions: the -10 region and the -35 region. The -10 region, also known as the Pribnow box, is the site where RNA polymerase binds to initiate transcription. The -35 region is another binding site for RNA polymerase, and is important for the recognition of the promoter by the enzyme.

In the LacUV5 promoter, the sequences of the -10 and -35 regions are altered compared to the wild-type lac promoter. These changes increase the binding affinity of RNA polymerase, leading to a higher level of transcription initiation.

Role in Research

The LacUV5 promoter has been widely used in molecular biology research due to its high level of activity and resistance to catabolite repression. It is often used in experiments that require high levels of gene expression, such as the production of recombinant proteins.

See Also

• Lac operon
• Promoter (genetics)
• Transcription (genetics)
• Catabolite repression

LacUV5 Resources
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