RNA polymerase

Enzyme that synthesizes RNA from a DNA template

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Structure of RNA polymerase

Identifiers
EC number	2.7.7.6
CAS number	9014-24-8
Alt. names
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway

RNA polymerase is an essential enzyme that synthesizes RNA from a DNA template, a process known as transcription. This enzyme is crucial for the expression of genes and the regulation of gene expression in all living organisms. RNA polymerase catalyzes the formation of the phosphodiester bonds between ribonucleotides, which are the building blocks of RNA.

Structure

RNA polymerase is a complex enzyme composed of multiple subunits. In prokaryotes, such as bacteria, the enzyme is relatively simple and consists of a core enzyme and a sigma factor. The core enzyme is responsible for the polymerization of RNA, while the sigma factor is necessary for the initiation of transcription.

In eukaryotes, RNA polymerase is more complex and exists in three main forms: RNA polymerase I, II, and III. Each type is responsible for transcribing different classes of genes. RNA polymerase II, for example, is responsible for transcribing mRNA, which is later translated into proteins.

Function

The primary function of RNA polymerase is to synthesize RNA by following a strand of DNA. The process begins with the binding of RNA polymerase to a specific region of DNA called the promoter. Once bound, the enzyme unwinds the DNA and begins synthesizing a complementary RNA strand by adding ribonucleotides that are complementary to the DNA template.

The process of transcription can be divided into three main stages:

• Initiation: RNA polymerase binds to the promoter region and begins unwinding the DNA.
• Elongation: The enzyme moves along the DNA template, adding ribonucleotides to the growing RNA chain.
• Termination: Transcription ends when RNA polymerase reaches a termination signal, causing the enzyme to release the newly synthesized RNA molecule.

Regulation

The activity of RNA polymerase is tightly regulated to ensure that genes are expressed at the right time and in the right amount. In prokaryotes, this regulation is often achieved through the use of operons, which are clusters of genes under the control of a single promoter. In eukaryotes, regulation is more complex and involves multiple factors, including transcription factors, enhancers, and silencers.

Clinical Significance

Mutations in RNA polymerase or its associated factors can lead to various diseases, including cancer. Additionally, some antibiotics, such as rifampicin, target bacterial RNA polymerase, making it an important target for antibacterial therapy.

Also see

• Transcription (biology)
• DNA polymerase
• Gene expression
• Ribosome
• RNA

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