Listeriolysin O

Listeriolysin O
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Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, which is the pathogen responsible for the infection known as listeriosis. LLO is a critical virulence factor that enables the bacteria to escape from the phagosome of the host cell into the cytoplasm, thereby evading the host's immune defense mechanisms.

Structure and Function

Listeriolysin O is a member of the cholesterol-dependent cytolysins (CDCs), a family of toxins that form pores in the membranes of host cells. The protein is encoded by the hly gene in Listeria monocytogenes. LLO is characterized by its ability to lyse phagosomal membranes at a low pH, facilitating the escape of Listeria from the phagosome into the cytoplasm of the host cell. This escape is essential for the replication of the bacteria and for spreading within the host.

Mechanism of Action

Upon infection, LLO plays a pivotal role during the intracellular life cycle of Listeria monocytogenes. The toxin is secreted by the bacteria and integrates into the phagosomal membrane. The acidic environment within the phagosome triggers a conformational change in LLO, activating its pore-forming activity. The resulting pores compromise the integrity of the phagosomal membrane, allowing the bacteria to access the host cell cytoplasm.

Clinical Significance

The presence and activity of LLO are directly linked to the pathogenicity of Listeria monocytogenes. Mutants lacking LLO are significantly less virulent, highlighting the importance of this toxin in listerial infections. Understanding the structure and function of LLO can aid in the development of targeted therapies or vaccines against listeriosis.

Research Applications

Research on Listeriolysin O has provided insights into the mechanisms of bacterial pathogenesis and host-pathogen interactions. Studies involving LLO have also contributed to the broader field of cellular microbiology, particularly in understanding how pathogens exploit host cell processes for infection and propagation.

See Also

• Listeria monocytogenes
• Listeriosis
• Hemolysin
• Virulence factor

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