Quellung reaction
Quellung Reaction
The Quellung Reaction or Quellung Phenomenon is a biochemical reaction that allows for the identification of certain bacteria species. This reaction is particularly useful in the identification of encapsulated bacteria, such as Streptococcus pneumoniae.
History[edit | edit source]
The Quellung Reaction was first described by the German bacteriologist Friedrich Neufeld and the Austrian physician Otto Plesch in the early 20th century. The term "Quellung" comes from the German word for "swelling", which describes the change in appearance of the bacteria when observed under a microscope during the reaction.
Mechanism[edit | edit source]
The Quellung Reaction involves the use of specific antiserum that contains antibodies against the capsular polysaccharide of the bacteria being tested. When the antiserum is added to a suspension of the bacteria, the antibodies bind to the capsular polysaccharide, causing it to swell and become more visible under the microscope. This change in appearance is known as the Quellung Reaction.
Applications[edit | edit source]
The Quellung Reaction is primarily used in the identification of encapsulated bacteria, particularly Streptococcus pneumoniae. It is also used in the identification of other bacteria species, such as Haemophilus influenzae and Neisseria meningitidis. The reaction is a valuable tool in microbiology and clinical pathology, as it allows for the rapid and accurate identification of bacteria species, which is crucial in the diagnosis and treatment of bacterial infections.
Limitations[edit | edit source]
While the Quellung Reaction is a valuable tool in the identification of bacteria, it does have its limitations. The reaction requires the use of specific antisera, which may not be readily available for all bacteria species. Additionally, the reaction is not always definitive, as some bacteria may not exhibit the characteristic swelling even when the appropriate antiserum is used.
See Also[edit | edit source]
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