Recombinant proteins

Recombinant Proteins are proteins that are derived from recombinant DNA technology. This technology involves the insertion of a DNA sequence coding for a protein of interest into a vector, which is then introduced into a host organism to produce the protein. Recombinant proteins are widely used in various fields such as medicine, research, and industry.

History

The production of recombinant proteins began in the 1970s with the development of recombinant DNA technology. The first recombinant protein to be produced was insulin, which was produced by Genentech in 1978. Since then, many other recombinant proteins have been produced, including growth hormones, clotting factors, and vaccines.

Production

The production of recombinant proteins involves several steps. First, the gene coding for the protein of interest is inserted into a plasmid, which is a small, circular piece of DNA. This plasmid is then introduced into a host organism, such as a bacterium or yeast, where it is replicated and expressed to produce the protein. The protein is then purified from the host organism.

Applications

Recombinant proteins have a wide range of applications. In medicine, they are used to produce vaccines and therapeutic proteins. In research, they are used to study protein function and structure. In industry, they are used to produce enzymes for various processes.

See Also

• Recombinant DNA
• Protein expression
• Genetic engineering

References

Recombinant proteins Resources
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