RefSeq

RefSeq or Reference Sequence is a project run by the National Center for Biotechnology Information (NCBI) that provides a curated collection of DNA sequences, RNA sequences, and protein sequences. The RefSeq project aims to provide a comprehensive, accurate, and up-to-date representation of reference sequences, which serve as a foundation for the functional elements of the genome.

Overview[edit | edit source]

The RefSeq project was initiated in 1999 by the NCBI, a part of the National Institutes of Health (NIH). The goal of the project is to provide a standard set of sequences to help annotate genes and genomes. These sequences are used as a baseline for research in genomics, proteomics, and bioinformatics.

RefSeq sequences are derived from the archival sequence data in GenBank, but they undergo additional curation and validation to ensure accuracy. The RefSeq database is updated regularly to incorporate new information from scientific research and genome sequencing projects.

Features[edit | edit source]

RefSeq provides several key features for researchers:

Curated Sequences: RefSeq sequences are manually curated by experts to ensure accuracy. This includes checking for errors, validating annotations, and updating sequences based on new research.

Comprehensive Coverage: RefSeq aims to include all known functional elements of a genome, including genes, proteins, non-coding RNAs, and pseudogenes.

Standardized Nomenclature: RefSeq uses a standardized naming system for sequences, making it easier for researchers to find and use the data.

Integration with Other Databases: RefSeq data is integrated with other NCBI databases, such as GenBank, PubMed, and BLAST, providing a comprehensive resource for genomic research.

Applications[edit | edit source]

RefSeq is widely used in biomedical research, including studies of gene expression, mutation analysis, comparative genomics, and genetic disease. It also serves as a reference for genome annotation projects and for the development of bioinformatics tools.