Uranyl acetate

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Uranyl Acetate

Uranyl acetate is a chemical compound with the formula UO2(CH3COO)2. It is a yellow-green crystalline solid that is commonly used in electron microscopy as a stain for biological samples. Uranyl acetate is known for its ability to enhance the contrast of biological specimens under the electron beam, making it a valuable tool in the field of microscopy.

Properties

Uranyl acetate is highly soluble in water and other polar solvents. It is a potent oxidizing agent and should be handled with care due to its radioactive properties. The compound has a characteristic yellow-green color and emits fluorescence under ultraviolet light.

Uses

Uranyl acetate is primarily used in electron microscopy to stain biological samples. When applied to specimens, it binds to nucleic acids and phospholipids, enhancing the contrast between different cellular structures. This allows researchers to visualize fine details within cells and tissues.

Safety

Due to its radioactivity, uranyl acetate poses health risks if not handled properly. Protective equipment, such as gloves and goggles, should be worn when working with this compound. Proper disposal methods must be followed to prevent environmental contamination.

See also

• Electron microscopy
• Staining (biology)