Amido black 10B

Amido Black 10B is a synthetic dye used primarily in the field of forensics and biochemistry. It is a staining agent that is used to detect proteins in various biological samples.

History

Amido Black 10B was first synthesized in the late 19th century. Its use as a protein stain was discovered in the early 20th century. Since then, it has been widely used in various scientific fields, including forensics, biochemistry, and molecular biology.

Properties

Amido Black 10B is a dark blue to black powder. It is soluble in water and alcohol, but insoluble in most organic solvents. It has a molecular weight of 616.59 g/mol and a chemical formula of C22H14N6Na2O9S2.

Uses

In forensics, Amido Black 10B is used to enhance fingerprints on porous surfaces, such as paper and wood. It is particularly effective at revealing fingerprints that have been washed or wiped away.

In biochemistry and molecular biology, Amido Black 10B is used as a staining agent to detect proteins in biological samples. It is often used in conjunction with other staining techniques, such as Coomassie Brilliant Blue and Silver staining, to provide a comprehensive analysis of protein samples.

Safety

Amido Black 10B is considered to be a low hazard substance. However, it can cause irritation to the eyes, skin, and respiratory tract if not handled properly. It is recommended to use personal protective equipment when handling this substance.

See Also

• Protein staining
• Forensic science
• Biochemistry
• Molecular biology