Aminooxyacetic acid

Aminooxyacetic acid (also known as AOAA) is a compound that is used in scientific research. It is an inhibitor of aminotransferases, enzymes that catalyze the transfer of an amino group from a donor molecule to a recipient molecule. AOAA is often used in studies investigating the role of these enzymes in various biological processes.

Chemistry

Aminooxyacetic acid is a carboxylic acid that contains an aminooxy functional group. This group, which consists of an amino group (NH2) bonded to an oxygen atom, is rare in nature but is a key feature of AOAA's chemical structure. The presence of this group allows AOAA to act as an inhibitor of aminotransferases.

Biological Activity

AOAA is a potent inhibitor of aminotransferases, a class of enzymes that play a crucial role in amino acid metabolism. These enzymes catalyze the transfer of an amino group from a donor molecule (usually an amino acid) to a recipient molecule. By inhibiting these enzymes, AOAA can disrupt normal amino acid metabolism, which can have a variety of effects on biological systems.

In addition to its role as an aminotransferase inhibitor, AOAA has also been found to inhibit the activity of cystathionine beta synthase, an enzyme involved in the metabolism of the amino acid methionine. This has led to its use in research into disorders of methionine metabolism, such as homocystinuria.

Uses in Research

Due to its ability to inhibit aminotransferases, AOAA is often used in scientific research. It is particularly useful in studies investigating the role of these enzymes in various biological processes, such as amino acid metabolism, neurotransmitter synthesis, and the regulation of nitrogen balance in the body.

For example, AOAA has been used in research into the role of aminotransferases in the synthesis of glutamate, a key neurotransmitter in the brain. By inhibiting the activity of these enzymes, researchers can study the effects of reduced glutamate production on brain function.

Safety

As with any chemical compound, care should be taken when handling AOAA. It is recommended that it be used only by trained professionals in a controlled laboratory environment. Potential hazards include skin and eye irritation, and potential harm if ingested or inhaled.

See Also

• Aminotransferase
• Amino acid metabolism
• Cystathionine beta synthase
• Homocystinuria