Brain heart infusion
Brain heart infusion (BHI) is a nutrient-rich medium used for the cultivation of a wide variety of microorganisms, including bacteria, yeasts, and molds. It is particularly useful in the cultivation of fastidious organisms that require enriched media for optimal growth.
Composition[edit | edit source]
Brain heart infusion is composed of several key ingredients that provide the necessary nutrients for microbial growth. These ingredients typically include:
- Calf brain infusion
- Beef heart infusion
- Peptone
- Dextrose
- Sodium chloride
- Disodium phosphate
- Distilled water
The exact composition can vary slightly depending on the manufacturer, but the essential components remain consistent to ensure the medium's effectiveness.
Preparation[edit | edit source]
To prepare BHI medium, the following steps are generally followed: 1. Measure the required amounts of each ingredient. 2. Dissolve the ingredients in distilled water. 3. Adjust the pH to the desired level, usually around 7.4. 4. Sterilize the medium by autoclaving at 121°C for 15 minutes. 5. Cool the medium to room temperature before use.
Applications[edit | edit source]
BHI is widely used in microbiology laboratories for various applications, including:
- Cultivation of Streptococcus species
- Growth of Listeria monocytogenes
- Isolation of fungi and yeasts
- Antibiotic susceptibility testing
- Research in microbial physiology and pathogenesis
Advantages[edit | edit source]
The advantages of using BHI medium include:
- Rich nutrient content that supports the growth of a wide range of microorganisms.
- Ability to cultivate fastidious organisms that may not grow well on other media.
- Versatility in various microbiological applications.
Limitations[edit | edit source]
Despite its advantages, BHI medium has some limitations:
- It may not be selective for specific microorganisms, leading to the growth of unwanted contaminants.
- The rich nutrient content can sometimes mask the presence of certain pathogens in mixed cultures.
See also[edit | edit source]
References[edit | edit source]
External links[edit | edit source]
| Brain heart infusion Resources | |
|---|---|
|
 |
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD
|
WikiMD's Wellness Encyclopedia |
| Let Food Be Thy Medicine Medicine Thy Food - Hippocrates |
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Medical Disclaimer: WikiMD is not a substitute for professional medical advice. The information on WikiMD is provided as an information resource only, may be incorrect, outdated or misleading, and is not to be used or relied on for any diagnostic or treatment purposes. Please consult your health care provider before making any healthcare decisions or for guidance about a specific medical condition. WikiMD expressly disclaims responsibility, and shall have no liability, for any damages, loss, injury, or liability whatsoever suffered as a result of your reliance on the information contained in this site. By visiting this site you agree to the foregoing terms and conditions, which may from time to time be changed or supplemented by WikiMD. If you do not agree to the foregoing terms and conditions, you should not enter or use this site. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.
Contributors: Prab R. Tumpati, MD
