Chemically defined media
Chemically defined media is a type of growth media used in microbiology and cell culture that contains only known, chemically defined components. This is in contrast to complex media, which contains a variety of nutrients and growth factors, but the exact composition is not known.
Composition[edit | edit source]
Chemically defined media typically contains a mixture of amino acids, vitamins, inorganic salts, and a carbon source. The exact composition can vary depending on the specific needs of the cells or organisms being cultured. For example, some cells may require additional lipids or trace elements.
Uses[edit | edit source]
Chemically defined media is often used in research and industrial applications where it is important to know the exact composition of the media. This can be important for studying the nutritional requirements of a specific organism, for producing recombinant proteins, or for growing cells for tissue engineering or cell therapy applications.
Advantages and Disadvantages[edit | edit source]
The main advantage of chemically defined media is that it allows for precise control over the nutrients and growth factors that the cells or organisms are exposed to. This can be important for experimental reproducibility and for understanding the effects of specific nutrients or growth factors.
However, chemically defined media can be more expensive and time-consuming to prepare than complex media. In addition, some cells or organisms may not grow as well in chemically defined media because they require additional nutrients or growth factors that are not included in the media.
See Also[edit | edit source]
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