DU 145

DU 145 is a human prostate cancer cell line used extensively in cancer research. It was derived from a brain metastasis of a prostate adenocarcinoma and is known for its utility in studying the mechanisms of prostate cancer progression and for testing potential anticancer drugs.

Characteristics

DU 145 cells are characterized by their epithelial morphology and their ability to grow in vitro. Unlike other prostate cancer cell lines such as LNCaP and PC-3, DU 145 cells are androgen-independent, meaning they do not require androgens for growth. This makes them particularly useful for studying advanced prostate cancer that no longer responds to androgen deprivation therapy.

Research Applications

DU 145 cells are used in a variety of research applications, including:

• Drug screening: Testing the efficacy of new chemotherapeutic agents.
• Genetic studies: Understanding the genetic mutations and pathways involved in prostate cancer.
• Cell signaling: Investigating the signaling pathways that drive cancer cell proliferation and survival.
• Metastasis: Studying the mechanisms by which prostate cancer cells spread to other parts of the body.

Culturing DU 145 Cells

DU 145 cells are typically cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. They are maintained at 37°C in a humidified atmosphere containing 5% carbon dioxide (CO2).

Related Cell Lines

• LNCaP
• PC-3
• 22Rv1

See Also

• Prostate cancer
• Cancer cell line
• Androgen independence
• Metastasis

References

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