Diphenylhexatriene

Diphenylhexatriene (DHP) is a fluorescent hydrocarbon compound that is commonly used in membrane research. It is a linear, unsaturated hydrocarbon consisting of a chain of six carbon atoms, with a phenyl group attached at each end.

Structure and Properties

Diphenylhexatriene has a linear structure, with the six carbon atoms in the middle forming a conjugated system. This means that the electrons in the pi bonds of this system are delocalized, which gives the molecule its fluorescent properties. The phenyl groups at each end of the molecule contribute to its hydrophobicity, which allows it to insert into lipid bilayers.

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Structure of Diphenylhexatriene

Uses in Research

Diphenylhexatriene is most commonly used in research as a fluorescent probe for studying the properties of lipid bilayers, such as their fluidity and phase behavior. When inserted into a lipid bilayer, the fluorescence of DHP is sensitive to the local environment, allowing researchers to gain insights into the properties of the membrane.

DHP is also used in anisotropy measurements, which can provide information about the orientation and mobility of molecules in a system. This can be particularly useful in studies of membrane proteins and their interactions with the lipid bilayer.

Safety and Handling

Like many hydrocarbons, Diphenylhexatriene is flammable and should be handled with care. It should be stored in a cool, dry place, away from sources of ignition. In case of contact with skin or eyes, rinse immediately with plenty of water and seek medical advice.

See Also

• Fluorescence
• Lipid bilayer
• Anisotropy