Embed

Embedding is a process in histology where tissue samples are encapsulated in a solid medium, such as paraffin wax or plastic resin, to allow thin sections to be cut and mounted on a microscope slide for examination. This process is crucial in the preparation of tissue samples for microscopy.

Process

The embedding process begins with the fixation of the tissue sample to preserve its structure. This is typically done using a fixative such as formalin. The tissue is then dehydrated, usually through a series of alcohol baths, to remove water. The dehydrated tissue is then cleared, which involves replacing the alcohol with a substance that is miscible with both alcohol and the embedding medium. The cleared tissue is then infiltrated with the embedding medium, which is subsequently hardened to form a block. The tissue can then be sectioned using a microtome.

Embedding Mediums

There are several different types of embedding mediums that can be used, each with their own advantages and disadvantages.

• Paraffin wax: This is the most commonly used embedding medium. It is easy to use and provides good preservation of tissue structure. However, it does not provide as fine a level of detail as some other mediums.

• Plastic resins: These provide a higher level of detail than paraffin wax, but are more difficult to use. They are typically used for electron microscopy.

• Frozen sections: In some cases, tissue may be frozen and sectioned without the use of an embedding medium. This is typically done for rapid diagnosis during surgery.

Applications

Embedding is used in a wide range of applications in both clinical and research settings. It is a crucial step in the preparation of tissue samples for histological examination, which is used in the diagnosis of many diseases, including cancer. It is also used in research to study the structure and function of tissues.

See Also

• Histology
• Microscopy
• Microtome
• Cancer

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