GTPgammaS

GTPgammaS

GTPgammaS (Guanosine 5'-O-[gamma-thio]triphosphate) is a non-hydrolyzable analog of GTP, which is resistant to the action of GTPase enzymes. It is commonly used in research to activate G-proteins in studies investigating signal transduction pathways.

Structure and Properties

GTPgammaS is a modified form of GTP, with a sulfur atom replacing one of the oxygen atoms in the gamma phosphate group. This modification renders the molecule resistant to hydrolysis by GTPase enzymes, allowing it to persist in the active state for a longer period of time.

Use in Research

In research, GTPgammaS is often used to activate G-proteins in studies investigating signal transduction pathways. When bound to GTPgammaS, G-proteins remain in the active state, allowing researchers to study the effects of prolonged G-protein activation.

GTPgammaS binding assays are a common method used to study G-protein coupled receptors (GPCRs). In these assays, GTPgammaS is used to measure the activity of GPCRs by monitoring the amount of GTPgammaS that is bound to the G-protein in response to receptor activation.

Safety and Handling

As with all laboratory chemicals, GTPgammaS should be handled with care. It should be stored in a cool, dry place and protected from light.

See Also

• Guanosine triphosphate
• G protein
• Signal transduction
• G protein-coupled receptor