Immunoelectrophoresis
Immunoelectrophoresis is a laboratory method used in biochemistry, immunology and other fields of science. This method combines two techniques, electrophoresis and immunodiffusion, to identify and measure proteins in a sample.
Overview[edit | edit source]
Immunoelectrophoresis is used to separate proteins based on their electrophoretic mobility and their reaction with specific antibodies. The process begins with electrophoresis, which separates proteins in a sample based on their size, shape, and electrical charge. After electrophoresis, the proteins are made to react with specific antibodies in a process known as immunodiffusion. This results in the formation of visible precipitin lines, which can be analyzed to identify and measure the proteins.
Applications[edit | edit source]
Immunoelectrophoresis has a wide range of applications in various fields of science. In clinical laboratories, it is used to diagnose and monitor diseases that affect protein levels in the body, such as multiple myeloma and autoimmune diseases. In research laboratories, it is used to study the properties of proteins and their interactions with antibodies.
Procedure[edit | edit source]
The procedure for immunoelectrophoresis involves several steps. First, a sample containing proteins is applied to a gel and subjected to electrophoresis. This separates the proteins based on their electrophoretic mobility. Next, the gel is overlaid with a layer of agarose containing specific antibodies. The proteins diffuse into the agarose and react with the antibodies, forming visible precipitin lines. These lines are then analyzed to identify and measure the proteins.
Limitations[edit | edit source]
While immunoelectrophoresis is a powerful tool for protein analysis, it has some limitations. The method requires specific antibodies for each protein of interest, which can be expensive and time-consuming to produce. Additionally, the method is not suitable for analyzing complex mixtures of proteins, as the precipitin lines can overlap and become difficult to interpret.
See Also[edit | edit source]
| Immunoelectrophoresis Resources | |
|---|---|
|
 |
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD
|
WikiMD's Wellness Encyclopedia |
| Let Food Be Thy Medicine Medicine Thy Food - Hippocrates |
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Medical Disclaimer: WikiMD is not a substitute for professional medical advice. The information on WikiMD is provided as an information resource only, may be incorrect, outdated or misleading, and is not to be used or relied on for any diagnostic or treatment purposes. Please consult your health care provider before making any healthcare decisions or for guidance about a specific medical condition. WikiMD expressly disclaims responsibility, and shall have no liability, for any damages, loss, injury, or liability whatsoever suffered as a result of your reliance on the information contained in this site. By visiting this site you agree to the foregoing terms and conditions, which may from time to time be changed or supplemented by WikiMD. If you do not agree to the foregoing terms and conditions, you should not enter or use this site. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.
Contributors: Prab R. Tumpati, MD
