Immunohistochemistry

Immunohistochemistry (IHC) is a method in pathology that uses the principle of antibodies binding specifically to antigens in biological tissues. This technique is widely used in the diagnosis of cancer, as it can demonstrate the presence and localization of specific proteins in tissues.

History

Immunohistochemistry was first described by Albert H. Coons in 1941 when he used a fluorescent tag to directly label antibodies to visualize pneumococcal antigens in infected tissues.

Methodology

The process of immunohistochemistry involves several steps. First, the tissue is fixed and embedded in paraffin to preserve the structure and proteins. The tissue is then sectioned and placed on a slide. The slide is then treated with antibodies that bind to the protein of interest. These antibodies can be visualized by a variety of methods, including fluorescence or enzymatic methods.

Applications

Immunohistochemistry is used in a wide range of applications, including:

• Cancer diagnosis: IHC can be used to identify the type and origin of cancer cells.
• Infectious disease diagnosis: IHC can be used to identify specific pathogens in tissue samples.
• Research: IHC is used in research to understand the distribution and localization of biomarkers and protein expression in different tissues.

Limitations

While immunohistochemistry is a powerful tool, it does have some limitations. These include potential issues with specificity and sensitivity, as well as the need for careful control experiments to ensure accurate interpretation of results.

See Also

• Histopathology
• Immunofluorescence
• Immunology
• Pathology

References

Immunohistochemistry Resources
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