MOLT-4

MOLT-4 is a human T lymphocyte cell line that is commonly used in biomedical research. It was first established in 1976 from the peripheral blood of a 19-year-old male with acute lymphoblastic leukemia (ALL). MOLT-4 cells are unique in that they have a high mitotic index, making them ideal for studies of cell division and cell cycle regulation.

Characteristics

MOLT-4 cells are immortalized, meaning they can divide indefinitely in culture. They are suspension cells, which means they do not attach to the surface of the culture dish. MOLT-4 cells are CD4+ T cells, a type of T cell that plays a key role in the immune system. They express the CD4 receptor, which allows them to recognize and respond to antigens presented by major histocompatibility complex (MHC) class II molecules on antigen-presenting cells.

Genetic Features

MOLT-4 cells have a complex karyotype, with numerous chromosomal aberrations. They are triploid, with three copies of most chromosomes. Notably, they carry a translocation between chromosomes 7 and 14, which results in the overexpression of the T cell receptor beta chain gene. This genetic alteration is thought to contribute to the leukemogenic potential of MOLT-4 cells.

Applications in Research

MOLT-4 cells are widely used in cancer research, particularly in studies of leukemia and lymphoma. They are also used in research on HIV and other viruses, due to their ability to be infected by these pathogens. In addition, MOLT-4 cells are used in studies of apoptosis, autophagy, and other forms of cell death.

See Also

• Cell line
• T lymphocyte
• Acute lymphoblastic leukemia
• CD4+ T cells

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