PC-12
PC-12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla. This cell line is widely used in neuroscience research due to its ability to differentiate into neuron-like cells in response to nerve growth factor (NGF).
Characteristics[edit | edit source]
PC-12 cells are characterized by their ability to stop proliferation and extend neurites when treated with NGF. This makes them a valuable model for studying neuronal differentiation and neurobiology.
Applications[edit | edit source]
PC-12 cells are used in various research applications, including:
- Neurotoxicity studies
- Signal transduction research
- Neuropharmacology
- Neurodegenerative disease models
Culture Conditions[edit | edit source]
PC-12 cells are typically cultured in RPMI 1640 medium supplemented with 10% horse serum and 5% fetal bovine serum. They require a humidified atmosphere with 5% carbon dioxide at 37°C.
Differentiation[edit | edit source]
Upon treatment with NGF, PC-12 cells undergo differentiation, characterized by the extension of neurites and the expression of neuronal markers. This process is used to study the mechanisms of neuronal development and regeneration.
See also[edit | edit source]
References[edit | edit source]
External links[edit | edit source]
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Contributors: Prab R. Tumpati, MD
