Radial immunodiffusion
Radial immunodiffusion (also known as Mancini method, Farr assay, or single radial immunodiffusion assay) is a common laboratory technique used to quantify the concentration of antigens (such as proteins) in a sample. The method was first described by Mancini, Carbonara, and Heremans in 1965.
Principle[edit | edit source]
Radial immunodiffusion involves the diffusion of antigens through a gel that contains specific antibodies. The antigen diffuses from a well into the gel, forming a circular precipitate. The diameter of this circle is directly proportional to the concentration of the antigen in the sample.
Procedure[edit | edit source]
The procedure for radial immunodiffusion involves several steps:
- A gel is prepared with a specific antibody.
- Wells are cut into the gel.
- The sample is added to the well.
- The gel is incubated to allow for antigen-antibody interaction.
- The diameter of the precipitate is measured.
Applications[edit | edit source]
Radial immunodiffusion is used in various fields, including:
- Immunology: to quantify specific proteins in a sample.
- Clinical pathology: to diagnose and monitor diseases.
- Research: to study antigen-antibody interactions.
Limitations[edit | edit source]
While radial immunodiffusion is a useful technique, it has several limitations:
- It is not suitable for measuring low concentrations of antigens.
- It requires specific antibodies for each antigen.
- It is time-consuming and requires careful handling.
See also[edit | edit source]
References[edit | edit source]
Radial immunodiffusion Resources | ||
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