Immunoprecipitation

Immunoprecipitation is a method that enables the purification of a protein. This technique is used to isolate a particular protein from a complex mixture using an antibody that specifically binds to that particular protein. This can be used to determine the presence and quantity of the protein of interest, and to identify other proteins that associate with it.

Procedure

The procedure of immunoprecipitation involves several steps. First, a sample containing a mixture of proteins is incubated with an antibody specific for the protein of interest. This forms an antibody-antigen complex. This complex is then precipitated out of the solution by the addition of a secondary reagent, often Protein A or G sepharose beads, which binds to the antibody. The precipitate is then washed to remove any non-specifically bound proteins, and the protein of interest is eluted from the beads.

Applications

Immunoprecipitation is used in a variety of applications, including the study of protein-protein interactions, the identification of proteins associated with a particular cellular structure, and the analysis of protein modifications.

See also

• Protein purification
• Antibody
• Protein A
• Protein G
• Sephadex

References

Immunoprecipitation Resources
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