TGBA01AD
Sucrose gap is a technique used in neurophysiology to study the electrical properties of excitable cells such as neurons and muscle cells. This technique involves replacing a portion of the extracellular fluid surrounding the cell with a solution of sucrose, which is a non-conducting substance. This creates a "gap" in the electrical conductivity around the cell, allowing researchers to measure changes in the cell's membrane potential.
History[edit | edit source]
The sucrose gap technique was first developed in the 1960s as a way to study the electrical properties of excitable cells. It has since been used in a wide range of research studies, including investigations into the mechanisms of action potentials, the properties of ion channels, and the effects of various drugs on cellular excitability.
Technique[edit | edit source]
In a typical sucrose gap experiment, a cell or tissue sample is placed in a chamber filled with an electrolyte solution. A portion of this solution is then replaced with a solution of sucrose, creating a "gap" in the electrical conductivity around the cell. Electrodes are placed on either side of the sucrose gap, allowing researchers to measure changes in the cell's membrane potential.
The sucrose gap technique has several advantages over other methods of studying cellular excitability. For example, it allows for the direct measurement of membrane potential changes, rather than relying on indirect methods such as voltage clamp or patch clamp techniques. It also allows for the study of cells in their natural, intact state, rather than requiring them to be broken apart or otherwise manipulated.
Applications[edit | edit source]
The sucrose gap technique has been used in a wide range of research studies. For example, it has been used to investigate the mechanisms of action potentials, the properties of ion channels, and the effects of various drugs on cellular excitability. It has also been used to study the electrical properties of various types of cells, including neurons, muscle cells, and cardiac cells.
See also[edit | edit source]
References[edit | edit source]
| TGBA01AD Resources | |
|---|---|
|
 |
Search WikiMD
Ad.Tired of being Overweight? Try W8MD's physician weight loss program.
Semaglutide (Ozempic / Wegovy and Tirzepatide (Mounjaro / Zepbound) available.
Advertise on WikiMD
|
WikiMD's Wellness Encyclopedia |
| Let Food Be Thy Medicine Medicine Thy Food - Hippocrates |
Translate this page: - East Asian
中文,
日本,
한국어,
South Asian
हिन्दी,
தமிழ்,
తెలుగు,
Urdu,
ಕನ್ನಡ,
Southeast Asian
Indonesian,
Vietnamese,
Thai,
မြန်မာဘာသာ,
বাংলা
European
español,
Deutsch,
français,
Greek,
português do Brasil,
polski,
română,
русский,
Nederlands,
norsk,
svenska,
suomi,
Italian
Middle Eastern & African
عربى,
Turkish,
Persian,
Hebrew,
Afrikaans,
isiZulu,
Kiswahili,
Other
Bulgarian,
Hungarian,
Czech,
Swedish,
മലയാളം,
मराठी,
ਪੰਜਾਬੀ,
ગુજરાતી,
Portuguese,
Ukrainian
Medical Disclaimer: WikiMD is not a substitute for professional medical advice. The information on WikiMD is provided as an information resource only, may be incorrect, outdated or misleading, and is not to be used or relied on for any diagnostic or treatment purposes. Please consult your health care provider before making any healthcare decisions or for guidance about a specific medical condition. WikiMD expressly disclaims responsibility, and shall have no liability, for any damages, loss, injury, or liability whatsoever suffered as a result of your reliance on the information contained in this site. By visiting this site you agree to the foregoing terms and conditions, which may from time to time be changed or supplemented by WikiMD. If you do not agree to the foregoing terms and conditions, you should not enter or use this site. See full disclaimer.
Credits:Most images are courtesy of Wikimedia commons, and templates Wikipedia, licensed under CC BY SA or similar.
Contributors: Prab R. Tumpati, MD
