Tyrocidine

Tyrocidine

Structures of Tyrocidine A-D

Tyrocidine is a cyclic peptide antibiotic produced by the bacterium Bacillus brevis. It is one of the major components of the antibiotic mixture known as tyrothricin, which also includes gramicidin. Tyrocidine is primarily effective against Gram-positive bacteria and is known for its ability to disrupt bacterial cell membranes.

Structure and Biosynthesis

The tyrocidine operon

Tyrocidine is composed of a cyclic decapeptide structure. The biosynthesis of tyrocidine is non-ribosomal, meaning it is synthesized by non-ribosomal peptide synthetases (NRPSs) rather than by the ribosome. The tyrocidine operon encodes the enzymes responsible for its synthesis. These enzymes include TycA, TycB, and TycC, which work together to assemble the peptide chain.

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Domain organization of Tyrocidine synthetase

The NRPSs involved in tyrocidine synthesis have a modular structure, with each module responsible for the incorporation of a specific amino acid into the growing peptide chain. The process involves the activation of amino acids, their transfer to carrier proteins, and subsequent peptide bond formation.

Mechanism of Action

Cyclization of Tyrocidine

Tyrocidine exerts its antibacterial effects by integrating into bacterial cell membranes, causing disruption and increased permeability. This leads to leakage of essential ions and molecules, ultimately resulting in cell death. The cyclic nature of tyrocidine allows it to form stable structures that can insert into lipid bilayers.

Hydrogen bonding in Tyrocidine

The ability of tyrocidine to form hydrogen bonds with membrane lipids is crucial for its function. This interaction destabilizes the membrane structure, making it an effective antibacterial agent.

Synthesis and Derivatives

Synthetic pathway of Tyrocidine

The synthesis of tyrocidine can be achieved through both natural biosynthetic pathways and chemical synthesis. Researchers have explored various derivatives of tyrocidine to enhance its antibacterial properties and reduce toxicity. Modifications to the peptide sequence can lead to changes in its spectrum of activity and stability.

Related Pages

• Gramicidin
• Bacillus brevis
• Non-ribosomal peptide synthetase
• Antibiotic

Tyrocidine

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  Structures of Tyrocidine A-D

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  Tyrocidine operon

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  Domain organization of Tyrocidine synthetase

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  Cyclization of Tyrocidine

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  Hydrogen bonding in Tyrocidine

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  Synthesis of Tyrocidine